AlerTox Sticks Fish: Reliable testing for fish
AlerTox Sticks Fish, a rapid and accurate test for the detection of fish proteins.
AlerTox Sticks (KT-6131 / KIT3038) kits are specially designed to help manufacturers keep their items free from the antigens that cause allergic reaction. It is user-friendly: no lab equipment or scientific knowledge required. It’s a must-have for any manufacturer or commercial kitchen that wants to ensure food safety. AlerTox Sticks Fish tests for fish proteins in solids and liquids. Alertox Sticks Fish Features:
- Quick: A reliable answer in less than 10 minutes
- Easy: No special equipment required; everything is included in the kit
- Reliable: Shelf-stable and long-lasting
- Cost efficient: competitive pricing and volume discounts available
- ELISA kits also available for 20+ allergens
Emport would be happy to answer your questions. Contact us for information on how the test works, and for ordering.
AlerTox Sticks kits are not suitable for home/consumer use.
What is a lateral flow test?
Lateral Flow Devices (or LFDs) refer to a category of test designed to detect the presence or absence of a particular substance, without the need for sophisticated lab equipment. Through capillary action, the sample is brought along the length of the test and – if the substance in question is detected – will cause a visible signal to appear. The most commonly-seen type of LFD test on the market today is the over-the-counter pregnancy test.
What allergens are AlerTox Sticks kits available for?
Interested in learning more about allergens and controlling in a manufacturing setting? Please refer to the articles on AlerTox in our collection.
Are there AlerTox ELISAs?
Yes, there are ELISA tests available on special order for all AlerTox Sticks allergens and many others including sesame, walnut, and coconut. Contact us for details.
Will I need any special equipment to run AlerTox tests?
Usually no, but some kits do require cotton swabs or cotton balls for surface testing.
How long does it take to run an AlerTox Sticks test?
Test results are available in less than 12 minutes.
What are AlerTox’s limits of detection?
Each AlerTox Sticks test kit has a slightly different LOD, but most can detect a minimum amount of allergen ranging from 1-10 ppm.
Does AlerTox Sticks hold any third-party validations?
AlerTox Sticks kits do not hold any external validation. However, no regulating body has established validations for any testing procedure for the allergens addressed by the suite of AlerTox Sticks kits. Please contact us if you are interested in receiving the validation reports.
What is the hook effect?
The kits are designed to detect traces of allergen, and therefore samples that are very high in allergenic proteins (eg more than 5,000-10,000 ppm) may incur a false negative in the form of a hook effect. Matrices that contain a very high amount of the allergen can override the test. If you suspect this to be the case, an option is to dilute the sample (1 part sample to 3 parts water, for example) and re-test. You can always contact us with questions.
Can I use AlerTox to test rinse water?
Yes, rinse water can be tested following the general instructions for testing liquids.
Can I use AlerTox to test surfaces?
Yes, the directions for testing surfaces is included in the manuals.
After the test was completed, the test strip showed a negative result (only the blue control line appeared). But when I checked the test strip the next day, I noticed a faint positive line. What’s going on?
The test results should be read at the time indicated in the manual for each allergen test (not before and not after). Any faint lines that appear after this time limit are not valid and do not indicate the presence of allergen in the sample. Conversely, positive results may fade in intensity after the time limit.
I got a test result that surprises me. What do I do?
If your results are unexpected, please email us with more information about your test. We’ll work with you until your questions are resolved.
I’m looking for information about best practices for allergen testing in my facility.
There aren’t any easy one-size-fits-all solutions to effectively control hazard points in the manufacturing process. Minimizing hazard points and identifying best practices takes experience and expertise.
Although we cannot offer help for developing your specific plan, we’ve collected some links we feel may be of interest. The FDA provides guidance documents that cover basic questions about compliance to FDA legislation. You can also check our blog for some practical applications for allergen control.
You can also reference Emport’s article Best Practices for Gluten-Free Manufacturers, which provides a fuller explanation of some of the key concepts in creating a hazard plan.
What are the legal requirements for labeling my food allergen-free?
Within the United States, FALCPA regulations cover the production and labeling of foods containing any of the eight currently listed major food allergens: soy, peanut, tree nut, wheat, egg, milk, crustacean/shellfish, and fish. Requirements in Canada can be found on Health Canada’s Food Allergen Labeling page. FARE, or Food Allergy Research and Education, also has detailed guidance documents for manufacturers of allergen-free foods.
Can AlerTox Sticks kits detect allergens in fermented or hydrolyzed food?
Lateral flow kits like AlerTox can have trouble with foods that have been fermented, hydrolyzed, malted, cured or otherwise very highly processed to the point where their proteins start to break down. The antibody in the test kit is looking for a very small sequence of amino acids, and it needs to have this sequence repeated at least 2x/molecule to catch and count the molecule in its search for the PPM value.
As food processing breaks the proteins apart, there is a phase where they could be too small to get “caught” by the test but still big enough to cause damage to someone with an allergy.
Additionally, it is very difficult to detect the allergen in oil, or the presence of oils from allergenic foods.
Additional troubleshooting per allergen:
The AlerTox Sticks Crustacean test is applicable for qualitative detection of target antigens in the samples of complex foods and surface swabs. The sensitivity of the test may decrease in case of heat-processed food or fat-rich samples (e.g. in presence of oil or cream). Some fish processing technologies, eg preparation of surimi, selectively deplete the antigen (tropomyosin), sometimes resulting in weak positive or even false negatives.
The AlerTox Sticks Egg kit recognizes Ovalbumin, which is a glycoprotein that comprises 54% of the total proteins of egg white. AlerTox Sticks Egg will not detect the allergen presence in samples that have been exposed to high heat for an extended period. (higher than 212°F for more than 30 minutes.)
It is impossible to manufacture a single kit that can recognize all species of fish, or all manufacturing conditions (boiled, in oil, in vinegar, dried etc). Although AlerTox Sticks Fish can recognize between 30-50 types of bony fish, some show a reduced response in certain manufacturing processes. Canned tuna and surimi, or crab stick, are particularly difficult to detect. Please contact us with information about the matrices you are testing. We’ll be able to help determine whether or not the kit will fit your needs.
AlerTox Sticks Mustard tests can detect mustard content of 1ppm, measured as ground raw unprocessed mustard seed. The test kit will detect the presence of all related plants, but will not detect the presence of mustard greens.
AlerTox Sticks Almond, Hazelnut, Walnut and Peanut
The sensitivity of all of the nut detection tests decrease in an environment rich in fats (for example, in the presence of oil or creams).
- AlerTox Sticks Almond shows a considerable cross-reactivity in pit extracts of the fruits of Prunus and related genuses: peach, plum and apricot. If these pits are crushed during industrial processing of fruit masses (for example, in the production of jam), the resulting material may show false positivity with AlerTox Sticks Almond.
- AlerTox Sticks Hazelnut does not detect hydrolyzed, low molecular weight fragments of the target protein and therefore it could show a false negative result with fermented hazelnut milk. The sensitivity of the test decreases in an environment rich in fats (for example, in the presence of oil or creams).
- AlerTox Sticks Peanut is not recommended for use with samples containing Brazil nut, buckwheat, or walnut. These ingredients can trigger a false positive. Additionally, samples containing more than 2% of the following ingredients require the use of skim milk powder and additional waiting time to ensure against false positives: cocoa, barley, rye, oat, lima bean, pumpkin seed, sesame, almond, coconut, hazelnut, macadamia, pistachio, pecan, pine nut, paprika, and lemon juice
AlerTox Sticks Sesame The sensitivity of the AlerTox Sticks Sesame test decreases in an environment rich in fats (for example, in the presence of oil or creams). The kit should not be used to detect sesame oil.
AlerTox Sticks Soy senses the presence of trypsin inhibitor from soybeans (STI) with a sensitivity of 100 ng / g (0.1 ppm). This inhibitor is denatured only in presence of high temperatures. There is no process to remove 100% of STI, although UHT processes significantly reduce the amounts of STI, making it undetectable to the kit. The kit should not be used to detect soy lecithin or soy oil.