What does Emport LLC do?
I own a business outside of the US. Do you ship to___?
Will you analyze my samples for gluten if I mail them to you?
I want to start producing gluten-free/allergen-free foods. Can you help?
What is a lateral flow test?
How sensitive are the GlutenTox tests?
According to the Codex Alimentarius Commission, the FDA and Health Canada, food can be considered “gluten-free” if it is made without gluten-containing ingredients and the amount of gluten present due to cross-contamination does not exceed 20 parts per million (ppm).
What is the G12 antibody?
G12 is able to recognize gluten in wheat, barley, rye and certain strains of oat (for more on oat toxicity, see question below). It is also uniquely well-suited to detecting gluten in hydrolyzed, fermented, or otherwise processed foods. In addition, the G12 antibody shows no cross-reactivity with soy or any other gluten-free matrices.
Emport is pleased to share some details from Biomedal regarding the G12 antibody, specifically developed to identify the 33-mer peptide of α- gliadin. Read more in our News post.
What kind of safety qualifications does GlutenTox have?
What is the GlutenTox Reader?
Is there a GlutenTox ELISA test?
Does GlutenTox make a good science fair project?
What is the expiration date of the kit?
At what temperature should I store the kit?
How should I dispose of the kit after use?
How do I test a food or beverage sample for gluten?
How do I test surfaces for gluten contamination, and what do the results mean?
Although it’s tempting to assign a ppm value for surface testing, this is technically impossible: after all, no one is eating the counter tops! Instead, surface gluten is measured in nanograms per square centimeter. The ppm value of a food that comes into contact with the surface would vary based on variables like volume (a little ball of dough or a big one?) and texture (dough vs an already-baked cookie?)
With GlutenTox, a negative surface test indicates that if a kilogram of absolutely gluten-free dough were kneaded on the surface, the dough would have
Click here for instructions, or scroll up one question. The instructions for testing surfaces for gluten begin at 2:59 on the video.
Does the G12 antibody recognize oat?
Are there items GlutenTox is not recommended for?
- Antioxidants, polyphenols, and tannins: Gluten found in matrices that are high in antioxidants, polyphenols, or tannins (for example cocoa or tea) can be difficult to extract. For this reason, it is possible that the amount of gluten present in these samples can be underestimated. If using GlutenTox Sticks, a separate polyphenol additive is available that can appropriately extract gluten from these matrices. Please note a positive result will always indicate the presence of gluten in the sample.
- Maggi – Seasoning – Arôme (liquid sauce): This seasoning sauce contains gluten as described by the manufacturer in its ingredients list. Note that its manufacturing process and other active ingredients are not suitable for GlutenTox Home. Consider this food as containing gluten.
- Soy Sauce: Naturally brewed soy sauce undergoes a fermentation process that often breaks down gluten to level close or below the detectable limit.
- Highly processed and complex matrices: Although GlutenTox Home and Pro tests have been successfully tested against a wide variety of highly-processed matrices, there may be some cases in which a more thorough extraction process is required to identify traces of gluten (for example, the multi-step extraction options available via GlutenTox Sticks). If you are planning to test a vitamin, supplement, medication, or other products that you feel may be highly processed, please contact us with more information about the specific item — we may recommend various modifications. In all cases, a positive test result always indicates the presence of gluten: to date no false positives have been found with G12.
- Items that are very high in silica: Large amounts silica can cause the extraction solution to “gel” in a way that can create challenges for the sample preparation process. These items often require special analysis in a laboratory setting. If you would like to test these items with GlutenTox, we suggest using half the amount of sample and double the number of drops as recommended in the manual.
- GlutenTox has been validated against some but not all personal care products. Because of the wide range of formulations and ingredients within personal care products, we recommend additional caution when testing shampoos, lotions, cosmetics, and other non-food items. While a positive result does always indicate the presence of gluten, false negatives are possible.
Can I test beer for gluten?
Below you’ll find some information relating to gluten detection in beers, including so-called ‘gluten-removed’ beers that use enzymes to degrade the malted barley.
- When it comes to testing for gluten in beer, antibody-based testing is currently the gold standard. An antibody is developed that looks for specific, short chains of amino acids that are unique to gluten (ie, not cross-reactive or found in other, safe foods). These short chains are called epitopes.
- Lateral flow / rapid kits require two copies of the chain – two epitopes – to “catch” the gluten molecule. For the G12 antibody, the epitope is found three times on the 33-mer peptide, which is known to be highly immunotoxic. So as long as the peptide is 2/3 intact, it should be identifiable.
- The most common type of lab test, a Sandwich ELISA, also requires two epitopes in order to identify gluten. There is a type of ELISA called a Direct or Competitive ELISA that only requires one epitope, but very few labs are trained to do these. They’re quite delicate and have not been validated as thoroughly as Sandwich ELISAs.
- There are situations where a beer could have small fragments of gluten in it that are too small for rapid tests to pick up (but that a Competitive ELISA could quantify). It’s generally accepted that these beers would not be celiac-safe.
- There are also situations where a beer could have small fragments of gluten that are too small for even a Competitive ELISA to pick up – and there is no consensus within the communities about whether or not these beers would be celiac-safe.
Can I test the oil in my deep fryer?
After 10 minutes, the test showed a negative result (only the blue control line appeared). But when I checked the test strip the next day, I noticed a faint pink line. What’s going on?
I'm looking for information about best practices for allergen testing in my facility
You can also reference Emport’s article Best Practices for Gluten-Free Manufacturers, which provides a fuller explanation of some of the key concepts in creating a hazard plan.
I got a test result that surprises me. What do I do?
For what allergens are AlerTox Sticks kits available?
Interested in learning more about allergens and controlling in a manufacturing setting? Please refer to the articles in our collection.
Are there AlerTox ELISAs?
Do you have resources for building an Allergen Control Plan?
What are the legal requirements for labeling my food allergen-free?
Will I need any special equipment to run AlerTox tests?
How long does it take to run an AlerTox Sticks test?
What are AlerTox's limits of detection?
Does AlerTox Sticks hold any third-party validations?
What is the hook effect?
Can AlerTox Sticks kits detect allergens in fermented or hydrolyzed food?
As food processing breaks the proteins apart, there is a phase where they could be too small to get “caught” by the test but still big enough to cause damage to someone with an allergy.
Need specific information about AlerTox Sticks Fish?
Can I use AlerTox to test rinse water?
Can I use AlerTox to test surfaces?
How is the OleoTest analysis conducted?
What is the expiration date on the kit?
How should OleoTest be stored?
How do I keep the tests as a record?
What kinds of oil can be tested?
- corn oil
- peanut oil
- soy oil
- sunflower oil
- palm oil
- canola oil
- vegetable oil (as a mixture of different food oils from the ones detailed above)
Oils used to fry heavily pigmented foods (such as red peppers) may require a different color chart/scale. Please contact us with questions about adjusting the scale for these other oils. OleoTest is not suitable for testing animal fats.
What does OleoTest measure?
What are polar compounds and why should I measure them?
Many countries carefully regulate the levels of polar compounds allowed in frying oil: