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Quantitative Allergen Detection

Detect and measure food allergens in ingredients, finished foods, and the manufacturing environment.

Whether your facility is dedicated to producing allergen free products, or includes lines shared with allergen containing recipes, identifying hazard points is your first step in managing the risk of cross contamination. A well-thought-out quality control plan takes experience and expertise to develop and to implement, but will result in benefits to your final product and ultimately the consumer.

Emport LLC offers several types of test kit to help identify food allergen cross-contact in incoming ingredients and finished goods, as well as in rinse water and on surfaces. Different test methods are suitable for different needs and environments.

When quantitative information is needed — in other words, when it is important to know precisely how much of a specific allergen is present — ELISA testing is the gold standard.

ELISA, or Enzyme-Linked ImmunoSorbent Assay, is a type of test that requires specialized lab equipment and solid lab skills. Testing can take anywhere from 1-4 hours, but will return a precise value, usually measured in PPM, for the analyte in question. Because tests should be run in duplicate and alongside premade standards, ELISA testing only truly becomes cost-efficient when testing several samples at a time.

If you’d like to learn more about test methodology, please read our article, “What’s the difference between LFD, PCR, and ELISA testing?

If you’re considering the switch to ELISA testing, our team will happily run a cost-benefit analysis for you.

Testing via third-party lab (ELISA and PCR) is available through our lab partners. Contact us for details and quotes.

ELISA Systems ELISA for allergens

The ELISA SYSTEMS assay is an enzyme-linked immunosorbent assay (ELISA) that may be used to screen food products for the presence of allergenic material caused by cross-contamination. This assay is a rapid and reliable test that significantly reduces the time required to screen food products and environmental samples for the presence of allergens.

  • Range of Quantification: 0.1 – 25ppm
  • Results in 60 – 75 minutes
  • 48 well kit
  • Store: between 2 – 8°C.
  • Note: swabs for environmental samples sold separately
  • Note: Additive for high-polyphenol matrices sold separately
  • Laboratory balance accurate to 2 decimal places.
  • Suitable clean containers for use in the sample extraction procedure. Low-binding capacity plastic disposable containers suggested.
  • Capped centrifuge test tubes (min 10 mL)
  • Test vials (1.5-2 mL)
  • Enzyme Conjugate and Substrate volumes prior to use.
  • Disposable gloves
  • Distilled or deionised water
  • Timer
  • Vortex mixer
  • Tube rotator (or similar mixing device)
  • Centrifuge
  • Thermostatically-controlled water bath
  • Automatic microplate washer (recommended)
  • Mono-channel pipettes, multi-channel pipettes (recommended), pipette tips
  • ELISA plate reader (with 450 nm filter)

Emport LLC is happy to provide a quote for any and all lab equipment recommended for use with with ELISA Gen 3 Gluten. We can work with you to build your quality control lab from the ground up, or supplement your existing lab facilities to enable you to begin using the test kit as part of your gluten-free safety protocol.

Shipping and ordering information


Kits require expedited shipping.
You will be emailed after purchase if we require additional information and/or shipping fee adjustments.

Kits should be refrigerated upon arrival. Failure to do so may result in decreased shelf life and/or sensitivity.

Orders only ship Monday- Wednesday.

Want a quote? Have questions? Contact us prior to ordering.

All sales are final. For more information, please see our Terms & Conditions.

Hygiena AlerToxTox® ELISA kits

The Hygiena AlerTox® ELISA tests are quantitative sandwich ELISAs designed for analyzing food, beverage, and surface samples for common allergens.

  • Results in 60-75 minutes (20-20-20 incubation)
  • 96 well kit for large number of samples
  • Store: 2 – 8 °C (36 – 46 °F).
  • Standards:5 standards including blank; Ready to use (except Casein and Milk).
  • Validated: Recovery rates, quantification range, and validated commodities available on request.
  • Note: Ovalbumin ELISA is validated for wine matrix only; Lysozyme is validated for wine and cheese matrices only.

he Hygiena AlerTox® ELISA tests are quantitative sandwich ELISAs designed for analyzing food, beverage, and surface samples for common allergens. An antibody directed against the target antigen (protein) is bound on the surface of a microtiter plate. Antigen-containing samples or standards are given into the wells of the microtiter plate. After 20 minutes incubation at room temperature (15 – 25 ºC/59 – 77 ºF), the wells are washed with diluted washing solution to remove unbound material. A peroxidase-conjugated second antibody directed against the same antigen is given into the wells and after 20 minutes of incubation the plate is washed again. A substrate solution is added and incubated for 20 minutes, resulting in the development of a blue color. The color development is inhibited by the addition of a Stop Solution, and the color turns yellow. The yellow color is measured photometrically at 450 nm. The concentration of the antigen is directly proportional to the color intensity of the test sample.

Not sure if this product is right for you? We love to chat, contact us with your questions.

  • Analytical scale (accurate to 0.1 g)
  • Capped centrifuge test tubes (min 10 mL)
  • Test vials (1.5-2 mL)
  • Disposable gloves
  • Distilled water
  • Timer
  • Vortex mixer
  • Tube rotator (or similar mixing device)
  • Centrifuge
  • Thermostatically-controlled water bath
  • Automatic microplate washer (recommended)
  • Mono-channel pipettes, multi-channel pipettes (recommended), pipette tips
  • ELISA plate reader (with 450 nm filter)

 

Shipping and ordering information


Kits require expedited shipping.
You will be emailed after purchase if we require additional information and/or shipping fee adjustments.

Kits should be refrigerated upon arrival. Failure to do so may result in decreased shelf life and/or sensitivity.

Orders only ship Monday- Wednesday.

Want a quote? Have questions? Contact us prior to ordering.

All sales are final. For more information, please see our Terms & Conditions.

Looking for Rapid Allergen Testing?

Lateral Flow Devices are fast and easy to use, without the need for sophisticated lab equipment. Emport maintains an extensive catalog of rapid testing kits.

Are you producing products with gluten?

We have a variety of rapid and ELISA test kits for more than two dozen common food allergens. Our team will be happy to point you in the right direction.