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GlutenTox® ELISA


SKU KT-6544 / KIT3075 Categories , , , , , ,


GlutenTox® ELISA Rapid G12

GlutenTox® ELISA Rapid G12 is a quantitative enzyme-linked immunosorbent assay (ELISA) designed for the determination of the immunotoxic fraction of gluten in food samples. The high specificity of the G12 and A1 antibodies used in this test allows this method to precisely quantify gluten in food, beverage, and rinse water samples. Also suitable for surface monitoring.

  • Limit of Detection: 0.4ppm gluten (products); 3.6 ng/cm2 gluten (surfaces)
  • Range of Quantification: 1.2 – 200ppm gluten
  • Results in 90 minutes
  • 96 well kit for large number of samples
  • Validated against a wide range of food matrices
  • No cross-reactivity
  • Not for hydrolyzed sources of gluten

AOAC License 042301 GlutenTox® ELISA Rapid G12 holds AOAC certification for detecting gluten in foods, license number 042301. Validation studies showed no cross-reactivity (false positives) and no false negatives, as well as 100% specificity and 100% sensitivity for food testing. The test was validated against these Food Matrices: Soy flour, corn bread, seasoning mix, rolled oats, evaporated milk and incurred bread matrix.

Shipping and information

Kits require expedited shipping. You will be emailed after purchase if we require additional information and/or shipping fee adjustments.

Kits should be refrigerated upon arrival. Failure to do so may result in decreased shelf life and/or sensitivity.

Orders only ship Monday- Wednesday.

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Necessary materials not supplied (but available for purchase from Emport LLC)

  • Analytical scale (accurate to 0.1 g)
  • Capped centrifuge test tubes (min 10 mL)
  • Test vials (1.5-2 mL)
  • Disposable gloves
  • Distilled water
  • Timer
  • Vortex mixer
  • Tube rotator (or similar mixing device)
  • Centrifuge
  • Thermostatically-controlled water bath
  • Automatic microplate washer (recommended)
  • Mono-channel pipettes, multi-channel pipettes (recommended), pipette tips
  • ELISA plate reader (with 450 nm filter)

Testing for gluten in foods that also contain polyphenols or antioxidants? The polyphenol pack is required for accurate quantification. This pack contains:

  • Polyphenol Additive Powder (25g)
  • Positive Control containing polyphenols (cocoa powder with gluten, 10 g)
  • Negative Control containing polyphenols (gluten-free cocoa powder, 10 g)

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