Detect and measure gluten in ingredients, finished foods, and the manufacturing environment.
Gluten, a protein found in wheat, barley, and rye, must be avoided by anyone with celiac disease or a gluten sensitivity. In many countries, foods that are labeled gluten-free must contain less than 20ppm of gluten. Additionally, wheat is a commonly-regulated food allergen around the globe.
Emport LLC offers several types of test kit to help identify gluten cross-contact in incoming ingredients and finished goods, as well as in rinse water and on surfaces. Different test methods are suitable for different needs and environments.
When quantitative information is needed — in other words, when it is important to know precisely how much gluten is present — ELISA testing is the gold standard. This is true for gluten as well as other common food allergens.
ELISA, or Enzyme-Linked ImmunoSorbent Assay, is a type of test that requires specialized lab equipment and solid lab skills. Testing can take anywhere from 1-4 hours, but will return a precise value, usually measured in PPM, for the analyte in question. Because tests should be run in duplicate and alongside premade standards, ELISA testing only truly becomes cost-efficient when testing several samples at a time.
If you’d like to learn more about test methodology, please read our article, “What’s the difference between LFD, PCR, and ELISA testing?“
If you’re considering the switch to ELISA testing, our team will happily run a cost-benefit analysis for you.
Testing via third-party lab (ELISA and PCR) is available through our lab partners. Contact us for details and quotes.
Hygiena GlutenTox® ELISA Rapid G12
GlutenTox® ELISA Rapid G12 is a sandwich ELISA designed to identify and quantify gluten in food samples. The high specificity of the G12 and A1 antibodies used in this test allows this method to precisely quantify gluten in food, beverage, and rinse water samples. Also suitable for surface monitoring.
- Limit of Detection (LOD): 0.4ppm gluten (products); 3.6 ng/cm2 gluten (surfaces)
- Range of Quantification (ROQ): 1.2 – 200ppm gluten
- Results in 90 minutes
- 96 well kit for large number of samples
- Validated against a wide range of food matrices
- No cross-reactivity
- Not for hydrolyzed sources of gluten
GlutenTox® ELISA Rapid G12 holds AOAC certification for detecting gluten in foods, license number 042301. Validation studies showed no cross-reactivity (false positives) and no false negatives, as well as 100% specificity and 100% sensitivity for food testing. The test was validated against these Food Matrices: Soy flour, corn bread, seasoning mix, rolled oats, evaporated milk and incurred bread matrix.
Not sure if this product is right for you? We love to chat, contact us with your questions.
- Analytical scale (accurate to 0.1 g)
- Capped centrifuge test tubes (min 10 mL)
- Test vials (1.5-2 mL)
- Disposable gloves
- Distilled water
- Timer
- Vortex mixer
- Tube rotator (or similar mixing device)
- Centrifuge
- Thermostatically-controlled water bath
- Automatic microplate washer (recommended)
- Mono-channel pipettes, multi-channel pipettes (recommended), pipette tips
- ELISA plate reader (with 450 nm filter)
Shipping and ordering information
Kits require expedited shipping. You will be emailed after purchase if we require additional information and/or shipping fee adjustments.
Kits should be refrigerated upon arrival. Failure to do so may result in decreased shelf life and/or sensitivity.
Orders only ship Monday- Wednesday.
Want a quote? Have questions? Contact us prior to ordering.
All sales are final. For more information, please see our Terms & Conditions.
The polyphenol pack is required for accurate quantification. This pack contains:
- Polyphenol Additive Powder (25g)
- Positive Control containing polyphenols (cocoa powder with gluten, 10 g)
- Negative Control containing polyphenols (gluten-free cocoa powder, 10 g)
Please note: this kit is not designed to quantify gluten in heavily hydrolyzed or fermented samples.
Did you know?
The G12 antibody found in all GlutenTox test kits recognizes the 33-mer peptide. This is the toxic fraction of gluten in wheat, barley, rye, and even sometimes oat (Structural basis for gluten intolerance in celiac sprue, Science, 2002).
ELISA Systems ELISA Gen 3 Gluten
The Generation 3 Gluten residue detection kit is an ELISA that has been optimised for the detection of both raw and cooked foods, nutritional supplements and environmental samples. It uses the 2D4 antibody to detect both native and deamidated gluten in wheat, barley, rye and their hybrids.
- Range of Quantification: 0.1 – 25ppm
- Results in 60 – 75 minutes
- 48 well kit
- Store: between 2 – 8°C.
- Note: swabs for environmental samples sold separately
- Note: Additive for high-polyphenol matrices sold separately
- Laboratory balance accurate to 2 decimal places.
- Suitable clean containers for use in the sample extraction procedure. Low-binding capacity plastic disposable containers suggested.
- Capped centrifuge test tubes (min 10 mL)
- Test vials (1.5-2 mL)
- Enzyme Conjugate and Substrate volumes prior to use.
- Disposable gloves
- Distilled or deionised water
- Timer
- Vortex mixer
- Tube rotator (or similar mixing device)
- Centrifuge
- Thermostatically-controlled water bath
- Automatic microplate washer (recommended)
- Mono-channel pipettes, multi-channel pipettes (recommended), pipette tips
- ELISA plate reader (with 450 nm filter)
Emport LLC is happy to provide a quote for any and all lab equipment recommended for use with with ELISA Gen 3 Gluten. We can work with you to build your quality control lab from the ground up, or supplement your existing lab facilities to enable you to begin using the test kit as part of your gluten-free safety protocol.
Shipping and ordering information
Kits require expedited shipping. You will be emailed after purchase if we require additional information and/or shipping fee adjustments.
Kits should be refrigerated upon arrival. Failure to do so may result in decreased shelf life and/or sensitivity.
Orders only ship Monday- Wednesday.
Want a quote? Have questions? Contact us prior to ordering.
All sales are final. For more information, please see our Terms & Conditions.
Of special note
Samples that have been subjected to excessive hydrolysis or fermentation may not be suitable for testing using this kit. This kit does not detect oat protein; however the ELISA Systems Oat Detection kit is suitable for that application.
Looking for Rapid Gluten Testing?
Lateral Flow Devices are fast and easy to use, without the need for sophisticated lab equipment. Emport maintains an extensive catalog of rapid testing kits.
Are you producing products with other allergens?
We have a variety of rapid and ELISA test kits for more than two dozen common food allergens. Our team will be happy to point you in the right direction.
